782 Characterization of mechano-biological pathways involved in bioprinted skin remodeling

Reorganization of fibrin is the first stage in matrix maturation process bioprinted skin and takes place over a few hours to days. Fibrin popular component many bioinks provisional extracellular present during wound healing. Cells embedded gels attach exert traction forces realign and/or degrade fibers, sense respond surrounding substrate mechanical feedback loop. Such cellular actions may remodel printed construct such way, rendering it no longer feasible for implantation. The goal this study understand based constructs better engineer 3D skin. Results show that fibroblasts hydrogels change viscoelastic properties networks at micron-scale, suggesting cells network mechanically interact. Rheological analysis revealed strain-stiffening pattern due non-linearly elastic forces, exhibiting when subjected large deformations. mRNA shows express elastin collagen within hydrogels. Phenotypic changes were also observed where became more elongated time. Overall, was found fiber alignment environment regulate cell behavior phenotype. addition constraint, as plastic frame around construct, significantly impacts remodeling compared those do not have any constraints. widely used bio-inks, therefore, information data obtained from these experiments can be mechanobiology cell-matrix interactions which has important implications understanding tissues.